Sensitive detection of Treponema pallidum by using the polymerase chain reaction.

We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybr...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Main Authors: Burstain, J M, Grimprel, E, Lukehart, S A, Norgard, M V, Radolf, J D
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1991
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269704/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1993770/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.1.62-69.1991
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