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Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA nicking activity

The number of strand-specific nicking endonucleases that are currently available for laboratory procedures and applications in vivo is limited, and none is sufficiently specific to nick single target sites within complex genomes. The extreme target specificity of homing endonucleases makes them attr...

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Autori principali: Niu, Yan, Tenney, Kristen, Li, Hongye, Gimble, Frederick S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2700736/
https://ncbi.nlm.nih.gov/pubmed/18644379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.07.010
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