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Multiplex Amplicon Genotyping by High-Resolution Melting

High-resolution amplicon melting is a simple method for genotyping that uses only generic PCR primers and a saturating DNA dye. Multiplex amplicon genotyping has previously been reported in a single color, but two instruments were required: a carousel-based rapid cycler and a high-resolution melting...

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Bibliographic Details
Main Authors: Seipp, Michael T., Durtschi, Jacob D., Voelkerding, Karl V., Wittwer, Carl T.
Format: Artigo
Language:Inglês
Published: Association of Biomolecular Resource Facilities 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2700465/
https://ncbi.nlm.nih.gov/pubmed/19568454
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