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Detection of rubella virus gene sequences by enzymatic amplification and direct sequencing of amplified DNA.

We developed a rapid and sensitive polymerase chain reaction (PCR) assay for detecting and identifying rubella virus (RV). A segment of the RV gene which encodes the E1 membrane glycoprotein of RV was selected as a target for PCR amplification. Single-stranded viral RNA, extracted from infected cell...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Eggerding, F A, Peters, J, Lee, R K, Inderlied, C B
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269913/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2056062/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.5.945-952.1991
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