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Application of a polymerase chain reaction for the detection of Mycobacterium leprae in skin tissues.

The polymerase chain reaction (PCR) based on the selective amplification of a 530-bp fragment of the gene encoding the proline-rich antigen of Mycobacterium leprae was applied on sections of fixed or frozen biopsy samples from leprosy patients. A simple procedure for the extraction of DNA from M. le...

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Udgivet i:J Clin Microbiol
Principais autores: de Wit, M Y, Faber, W R, Krieg, S R, Douglas, J T, Lucas, S B, Montreewasuwat, N, Pattyn, S R, Hussain, R, Ponnighaus, J M, Hartskeerl, R A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1991
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269906/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2056057/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.5.906-910.1991
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