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Detection of human immunodeficiency virus type 1 DNA by polymerase chain reaction amplification and capture hybridization in microtiter wells.

We developed an improved microtiter-based assay for the detection of polymerase chain reaction (PCR)-amplified DNA sequences. The synthetic DNA sequences used to prime the PCR were labeled with biotin at their 5' ends so that the specific PCR product was labeled with biotin. Following amplification,...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Keller, G H, Huang, D P, Manak, M M
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269835/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2037686/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.3.638-641.1991
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