Detection of human immunodeficiency virus type 1 DNA by polymerase chain reaction amplification and capture hybridization in microtiter wells.

We developed an improved microtiter-based assay for the detection of polymerase chain reaction (PCR)-amplified DNA sequences. The synthetic DNA sequences used to prime the PCR were labeled with biotin at their 5' ends so that the specific PCR product was labeled with biotin. Following amplification,...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Keller, G H, Huang, D P, Manak, M M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1991
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269835/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2037686/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.3.638-641.1991
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