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Using Morpholinos for Gene Knockdown in Giardia intestinalis
We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2698301/ https://ncbi.nlm.nih.gov/pubmed/19377039 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00041-09 |
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