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Rapid Tagging and Integration of Genes in Giardia intestinalis
We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using th...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3019802/ https://ncbi.nlm.nih.gov/pubmed/21115739 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00190-10 |
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