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The rough energy landscape of superfolder GFP is linked to the chromophore

Many GFP variants have been developed for use as fluorescent tags, and recently a superfolder GFP (sfGFP) has been developed as a robust folding reporter. This new variant shows increased stability and improved folding kinetics, as well as 100% recovery of native protein after denaturation. Here, we...

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Detalhes bibliográficos
Main Authors: Andrews, Benjamin T., Schoenfish, Andrea R., Roy, Melinda, Waldo, Geoffrey, Jennings, Patricia A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2695656/
https://ncbi.nlm.nih.gov/pubmed/17822714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.07.071
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