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Fluorophore-Quencher Pair For Monitoring Protein Motion
A fluorophore/quencher pair capable of detecting conformational changes of DNA-protein complexes is described. The system employs a fluorescent nucleoside analog 1,3-diaza-2-oxophenothiazine (tC) within duplex DNA and a non-fluorescent quencher (TEMPO) attached to an engineered cysteine residue of t...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2693715/ https://ncbi.nlm.nih.gov/pubmed/19167347 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2009.01.055 |
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