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Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei
Genetic manipulation in African trypanosomes typically relies upon electroporation with chromosomal integration of DNA constructs by homologous recombination. Relatively little is known about chromosomal recombination and repair in these organisms however and low transformation efficiency and positi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier/North-Holland Biomedical Press
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2691778/ https://ncbi.nlm.nih.gov/pubmed/19459229 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2009.03.010 |
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