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Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning

To accelerate gene isolation from plants by positional cloning, vector systems suitable for both chromosome walking and genetic complementation are highly desirable. Therefore, we developed a transformation-competent artificial chromosome (TAC) vector, pYLTAC7, that can accept and maintain large gen...

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Autors principals: Liu, Yao-Guang, Shirano, Yumiko, Fukaki, Hidehiro, Yanai, Yukihiro, Tasaka, Masao, Tabata, Satoshi, Shibata, Daisuke
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC26917/
https://ncbi.nlm.nih.gov/pubmed/10339623
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