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Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a...

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Detalhes bibliográficos
Main Authors: Barletta, F., Ochoa, T. J., Ecker, L., Gil, A. I., Lanata, C. F., Cleary, T. G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2691103/
https://ncbi.nlm.nih.gov/pubmed/19357211
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00608-09
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