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Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples

BACKGROUND: A fast and simple two-step multiplex real-time PCR assay has been developed to replace the traditional, laborious Salmonella serotyping procedure. Molecular beacons were incorporated into the assay as probes for target DNA. Target sequences were regions of the invA, prot6E and fliC genes...

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Main Authors: Hadjinicolaou, Andreas V, Demetriou, Victoria L, Emmanuel, Maria A, Kakoyiannis, Charalambos K, Kostrikis, Leondios G
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2689230/
https://ncbi.nlm.nih.gov/pubmed/19454003
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-9-97
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