A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells.

We provide our detailed, standardized in vitro protocol for culture and differentiation of human retinal pigment epithelial (RPE) cells into a highly polarized, functional monolayer. Disruption of polarized RPE function plays an important role in the pathogenesis of common blinding disorders of the...

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Main Authors: Sonoda, Shozo, Spee, Christine, Barron, Ernesto, Ryan, Stephen J, Kannan, Ram, Hinton, David R
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2688697/
https://ncbi.nlm.nih.gov/pubmed/19373231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.33
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