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Accurate characterization of weak macromolecular interactions by titration of NMR residual dipolar couplings: application to the CD2AP SH3-C:ubiquitin complex

The description of the interactome represents one of key challenges remaining for structural biology. Physiologically important weak interactions, with dissociation constants above 100 μM, are remarkably common, but remain beyond the reach of most of structural biology. NMR spectroscopy, and in part...

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Bibliografski detalji
Glavni autori: Ortega-Roldan, Jose Luis, Jensen, Malene Ringkjøbing, Brutscher, Bernhard, Azuaga, Ana I., Blackledge, Martin, van Nuland, Nico A. J.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2685109/
https://ncbi.nlm.nih.gov/pubmed/19359362
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp211
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