Optimization and Validation of an 8-Color Intracellular Cytokine Staining (ICS) Assay to Quantify Antigen-Specific T Cells Induced by Vaccination

Candidate HIV-1 vaccines currently being evaluated in clinical trials are designed to elicit HIV-1-specific cellular immunity. Intracellular cytokine staining (ICS) assays allow sensitive, quantitative ex vivo assessments of antigen-specific T cells including immunophenotyping of responding cells an...

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Detalhes bibliográficos
Main Authors: Horton, Helen, Thomas, Evan, Stucky, Jason, Frank, Ian, Moodie, Zoe, Huang, Yunda, Chiu, Ya-Lin, McElrath, M. Juliana, De Rosa, Stephen C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2683732/
https://ncbi.nlm.nih.gov/pubmed/17451739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.03.002
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