Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies

Vaccination and SIV challenge of macaque species is the best animal model for evaluating candidate HIV vaccines in pre-clinical studies. As such, robust assays optimized for use in nonhuman primates are necessary for reliable ex vivo measurement of immune responses and identification of potential im...

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Main Authors: Donaldson, Mitzi M., Kao, Shing-Fen, Eslamizar, Leila, Gee, Connie, Koopman, Gerrit, Lifton, Michelle, Schmitz, Joern E., Sylwester, Andrew W., Wilson, Aaron, Hawkins, Natalie, Self, Steve G., Roederer, Mario, Foulds, Kathryn E.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3646372/
https://ncbi.nlm.nih.gov/pubmed/22955212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2012.08.011
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