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Continuous axenic cultivation of Pneumocystis carinii

Continuous axenic culture of Pneumocystis carinii has been achieved. A culture vessel is used that allows for frequent medium exchange without disturbance of organisms that grow attached to a collagen-coated porous membrane. The growth medium is based on Minimal Essential Medium with Earle’s salt su...

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Main Authors: Merali, Salim, Frevert, Ute, Williams, Jonathan H., Chin, Kevin, Bryan, Richard, Clarkson, Allen B.
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences 1999
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC26796/
https://ncbi.nlm.nih.gov/pubmed/10051654
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