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Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.
A method was developed for the purification of rotavirus RNA from fecal extracts in order to permit the sensitive identification of group A rotavirus in fecal specimens by the polymerase chain reaction. Sequential reactions with reverse transcriptase and Taq polymerase with directed primers from rot...
Bewaard in:
Hoofdauteurs: | , , |
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Formaat: | Artigo |
Taal: | Inglês |
Gepubliceerd in: |
1990
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Onderwerpen: | |
Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC267924/ https://ncbi.nlm.nih.gov/pubmed/1696283 |
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