Llwytho...

Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization

The effects of photocaged nucleosides on the DNA polymerization reaction was investigated, finding that most polymerases are unable to recognize and read through the presence of a single caging group on the DNA template. Based on this discovery, a new method of introducing mutations into plasmid DNA...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Young, Douglas D., Lusic, Hrvoje, Lively, Mark O., Deiters, Alexander
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2677887/
https://ncbi.nlm.nih.gov/pubmed/19293272
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp150
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