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Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple a...

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Detaylı Bibliyografya
Asıl Yazarlar: Sekar, Raju, Kaczmarsky, Longin T., Richardson, Laurie L.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 2009
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2675240/
https://ncbi.nlm.nih.gov/pubmed/19251896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01500-08
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