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Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple a...

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Detalhes bibliográficos
Main Authors: Sekar, Raju, Kaczmarsky, Longin T., Richardson, Laurie L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2675240/
https://ncbi.nlm.nih.gov/pubmed/19251896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01500-08
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