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Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology

The capillary electrophoresis (CE) conditions for a competitive immunoassay of glucagon were optimized for highest sensitivity of the immunoassay and resolution of the electrophoretic peaks using a Box-Behnken design. Injection time, voltage ramp time, and separation voltage were varied between thre...

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Detalhes bibliográficos
Main Authors: Lomasney, Anna R., Guillo, Christelle, Sidebottom, Ashley M., Roper, Michael G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2667558/
https://ncbi.nlm.nih.gov/pubmed/19189083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-009-2622-2
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