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Optimization of capillary electrophoresis conditions for a glucagon competitive immunoassay using response surface methodology

The capillary electrophoresis (CE) conditions for a competitive immunoassay of glucagon were optimized for highest sensitivity of the immunoassay and resolution of the electrophoretic peaks using a Box-Behnken design. Injection time, voltage ramp time, and separation voltage were varied between thre...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lomasney, Anna R., Guillo, Christelle, Sidebottom, Ashley M., Roper, Michael G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2667558/
https://ncbi.nlm.nih.gov/pubmed/19189083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-009-2622-2
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