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Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease

Homing endonucleases stimulate gene conversion by generating site-specific DNA double-strand breaks that are repaired by homologous recombination. These enzymes are potentially valuable tools for targeted gene correction and genome engineering. We have engineered a variant of the I-AniI homing endon...

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書誌詳細
主要な著者: McConnell Smith, Audrey, Takeuchi, Ryo, Pellenz, Stefan, Davis, Luther, Maizels, Nancy, Monnat, Raymond J., Stoddard, Barry L.
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2009
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オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664052/
https://ncbi.nlm.nih.gov/pubmed/19276110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0810588106
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