Construction of Disarmed Ti Plasmids Transferable between Escherichia coli and Agrobacterium Species

Agrobacterium-mediated plant transformation has been used widely, but there are plants that are recalcitrant to this type of transformation. This transformation method uses bacterial strains harboring a modified tumor-inducing (Ti) plasmid that lacks the transfer DNA (T-DNA) region (disarmed Ti plas...

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Detalhes bibliográficos
Main Authors: Kiyokawa, Kazuya, Yamamoto, Shinji, Sakuma, Kei, Tanaka, Katsuyuki, Moriguchi, Kazuki, Suzuki, Katsunori
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663228/
https://ncbi.nlm.nih.gov/pubmed/19181833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01856-08
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