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Successful Transfer of a Model T-DNA Plasmid to E. coli Revealed Its Dependence on Recipient RecA and the Preference of VirD2 Relaxase for Eukaryotes Rather Than Bacteria as Recipients

In Agrobacterium-mediated transformation (AMT) of plants, a single-strand (ss) T-DNA covalently linked with a VirD2 protein moves through a bacterial type IV secretion channel called VirB/D4. This transport system originates from conjugal plasmid transfer systems of bacteria. The relaxase VirD2 and...

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Bibliographische Detailangaben
Veröffentlicht in:Front Microbiol
Hauptverfasser: Ohmine, Yuta, Kiyokawa, Kazuya, Yunoki, Kazuya, Yamamoto, Shinji, Moriguchi, Kazuki, Suzuki, Katsunori
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5985610/
https://ncbi.nlm.nih.gov/pubmed/29892270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.00895
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