High-Throughput Bioluminescence-Based Mutant Screening Strategy for Identification of Bacterial Virulence Genes

A high-throughput bioluminescence screening procedure for identification of virulence genes in bacteria was developed and applied to the fish pathogen Edwardsiella ictaluri. A random transposon mutant library expressing bioluminescence was constructed and robotically arrayed on 384-well plates. Muta...

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Bibliographic Details
Main Authors: Karsi, Attila, Gülsoy, Nagihan, Corb, Erin, Dumpala, Pradeep R., Lawrence, Mark L.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2009
Subjects:
Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663204/
https://ncbi.nlm.nih.gov/pubmed/19201969
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02449-08
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