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High-Throughput Bioluminescence-Based Mutant Screening Strategy for Identification of Bacterial Virulence Genes
A high-throughput bioluminescence screening procedure for identification of virulence genes in bacteria was developed and applied to the fish pathogen Edwardsiella ictaluri. A random transposon mutant library expressing bioluminescence was constructed and robotically arrayed on 384-well plates. Muta...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2663204/ https://ncbi.nlm.nih.gov/pubmed/19201969 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02449-08 |
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