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Quantitative and Label-Free Technique for Measuring Protease Activity and Inhibition using a Microfluidic Cantilever Array

We report the use of a SiN(x) based gold coated microcantilever array to quantitatively measure the activity and inhibition of a model protease immobilized on its surface. Trypsin was covalently bound to the gold surface of the microcantilever using a synthetic spacer, and the remaining exposed sili...

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Detalhes bibliográficos
Main Authors: Raorane, Digvijay A., Lim, Mark D., Chen, Fanqing Frank, Craik, Charles S., Majumdar, Arun
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663003/
https://ncbi.nlm.nih.gov/pubmed/18720973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl8019455
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