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Exploring writhe in supercoiled minicircle DNA
Using λ-Int recombination in E. coli, we have generated milligram quantities of supercoiled minicircle DNA. Intramolecular Int recombination was efficient down to lengths ~254 bp. When nicked and religated in the presence of ethidium bromide, 339 bp minicircles adopted at least seven unique topoisom...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2662687/ https://ncbi.nlm.nih.gov/pubmed/19337583 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1088/0953-8984/18/14/S01 |
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