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Catalytic Mechanism of Nitrile Hydratase Proposed by Time-resolved X-ray Crystallography Using a Novel Substrate, tert-Butylisonitrile

Nitrile hydratases (NHases) have an unusual iron or cobalt catalytic center with two oxidized cysteine ligands, cysteine-sulfinic acid and cysteine-sulfenic acid, catalyzing the hydration of nitriles to amides. Recently, we found that the NHase of Rhodococcus erythropolis N771 exhibited an additiona...

תיאור מלא

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מידע ביבליוגרפי
Main Authors: Hashimoto, Koichi, Suzuki, Hiroyuki, Taniguchi, Kayoko, Noguchi, Takumi, Yohda, Masafumi, Odaka, Masafumi
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Biochemistry and Molecular Biology 2008
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2662313/
https://ncbi.nlm.nih.gov/pubmed/18948265
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M806577200
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