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Nonenzymatic RNA Ligation in Water
We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short R...
में बचाया:
| मुख्य लेखकों: | , , , |
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| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
American Society for Biochemistry and Molecular Biology
2008
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2662306/ https://ncbi.nlm.nih.gov/pubmed/18977755 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M805333200 |
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