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Nonenzymatic RNA Ligation in Water

We describe the nonenzymatic ligation of RNA oligomers in water. Dimers and tetramers are formed in a time-, pH-, and temperature-dependent reaction. Ligation efficiency depends on oligonucleotide length and sequence and is strongly enhanced by adenine-based nucleotide cofactors. Ligation of short R...

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Detalhes bibliográficos
Main Authors: Pino, Samanta, Ciciriello, Fabiana, Costanzo, Giovanna, Di Mauro, Ernesto
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2662306/
https://ncbi.nlm.nih.gov/pubmed/18977755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M805333200
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