Evaluation of new enzyme-linked immunosorbent assay based on a supernatant containing Staphylococcus aureus alpha-toxin produced by Bacillus subtilis.
The gene encoding alpha-toxin from Staphylococcus aureus was cloned into a Bacillus subtilis expression vector (pEF 231/alpha-Tox). The protease-deficient B. subtilis strain DB 104 transformed with pEF 231/alpha-Tox expressed and secreted 5 mg of alpha-toxin per liter into the growth medium. The alp...
Enregistré dans:
| Publié dans: | J Clin Microbiol |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1993
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC266202/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8263193/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.11.3036-3039.1993 |
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