Evaluation of new enzyme-linked immunosorbent assay based on a supernatant containing Staphylococcus aureus alpha-toxin produced by Bacillus subtilis.

The gene encoding alpha-toxin from Staphylococcus aureus was cloned into a Bacillus subtilis expression vector (pEF 231/alpha-Tox). The protease-deficient B. subtilis strain DB 104 transformed with pEF 231/alpha-Tox expressed and secreted 5 mg of alpha-toxin per liter into the growth medium. The alp...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Egnell, P, Christensson, B, Möllby, R, Flock, J I
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC266202/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8263193/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.11.3036-3039.1993
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