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Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection.

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-...

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Udgivet i:J Clin Microbiol
Principais autores: Tamburrini, E, Mencarini, P, De Luca, A, Antinori, A, Visconti, E, Ammassari, A, Ortona, L, Ortona, E, Siracusano, A, Vicari, G
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC266016/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8253985/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.31.10.2788-2789.1993
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