Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection.

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-...

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Detalhes bibliográficos
Main Authors: Tamburrini, E, Mencarini, P, De Luca, A, Antinori, A, Visconti, E, Ammassari, A, Ortona, L, Ortona, E, Siracusano, A, Vicari, G
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC266016/
https://ncbi.nlm.nih.gov/pubmed/8253985
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