Selecting Optimum Eukaryotic Integral Membrane Proteins for Structure Determination by Rapid Expression and Solubilization Screening

A medium throughput approach is used to rapidly identify membrane proteins from a eukaryotic organism that are most amenable to expression in amounts and quality adequate to support structure determination. The goal was to expand knowledge of new membrane protein structures based on proteome-wide co...

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Hauptverfasser: Li, Min, Hays, Franklin A., Roe-Zurz, Zygy, Vuong, Linda, Kelly, Libusha, Ho, Chi-Min, Robbins, Renée M., Pieper, Ursula, O’Connell, Joseph D., Miercke, Larry J. W., Giacomini, Kathleen M., Sali, Andrej, Stroud, Robert M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2659619/
https://ncbi.nlm.nih.gov/pubmed/19061901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.11.021
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