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Development of oligonucleotide primers and probes against structural and regulatory genes of human immunodeficiency virus type 1 (HIV-1) and their use for amplification of HIV-1 provirus by using polymerase chain reaction.

The polymerase chain reaction is a powerful technique for amplifying a few copies of double-stranded genetic material to millions of copies in a few hours. The sensitivity and specificity of the polymerase chain reaction technique depend to some extent on the nucleotide sequences of the oligonucleot...

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Publicat a:J Clin Microbiol
Autors principals: Dawood, M R, Allan, R, Fowke, K, Embree, J, Hammond, G W
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1992
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265492/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1400991/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.9.2279-2283.1992
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