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Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene.

Synthetic oligonucleotide primers of 21 and 24 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the nuc gene, which encodes the thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from lysed S. aureus cell...

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Библиографические подробности
Опубликовано в: :J Clin Microbiol
Главные авторы: Brakstad, O G, Aasbakk, K, Maeland, J A
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology (ASM) 1992
Предметы:
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265359/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629319/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.7.1654-1660.1992
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