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Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene.
Synthetic oligonucleotide primers of 21 and 24 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the nuc gene, which encodes the thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from lysed S. aureus cell...
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| Publicado en: | J Clin Microbiol |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1992
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265359/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1629319/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.7.1654-1660.1992 |
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