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Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity

BACKGROUND: Endonuclease G (EndoG), a member of DNA/RNA nonspecific ββα-Me-finger nucleases, is involved in apoptosis and normal cellular proliferation. In this study, we analyzed the critical amino acid residues of EndoG and proposed the catalytic mechanism of EndoG. METHODS: To identify the critic...

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Detalhes bibliográficos
Main Authors: Wu, Shih-Lu, Li, Chia-Cheng, Chen, Jaw-Chyun, Chen, Yi-Jin, Lin, Ching-Ting, Ho, Tin-Yun, Hsiang, Chien-Yun
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2653514/
https://ncbi.nlm.nih.gov/pubmed/19272175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1423-0127-16-6
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