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Crystallization and preliminary characterization of the Thermus thermophilus RNA helicase Hera C-­terminal domain

Heat-resistant RNA-dependent ATPase (Hera) from Thermus thermophilus is a DEAD-box RNA helicase. Two constructs encompassing the second RecA-like domain and the C-terminal domain of Hera were overproduced in Escherichia coli and purified to homogeneity. Single crystals of both Hera constructs were o...

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Detalhes bibliográficos
Main Authors: Rudolph, Markus G., Wittmann, Julia G., Klostermeier, Dagmar
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2650459/
https://ncbi.nlm.nih.gov/pubmed/19255475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108043145
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