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Ovalbumin blocking improves sensitivity and specificity of immunoglobulin M immunoblotting for serodiagnosis of patients with erythema migrans.

To improve the serodiagnosis of erythema migrans, we evaluated how sensitivity and specificity of immunoblotting are influenced by antigen concentration and blocking conditions. We found that an antigen concentration of 0.5 micrograms per lane in concert with ovalbumin blocking of the nitrocellulose...

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Bibliografische gegevens
Gepubliceerd in:J Clin Microbiol
Hoofdauteurs: Lange, R, Bocklage, H, Schneider, T, Kölmel, H W, Heesemann, J, Karch, H
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1992
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265029/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1734059/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.1.229-232.1992
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