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Ovalbumin blocking improves sensitivity and specificity of immunoglobulin M immunoblotting for serodiagnosis of patients with erythema migrans.

To improve the serodiagnosis of erythema migrans, we evaluated how sensitivity and specificity of immunoblotting are influenced by antigen concentration and blocking conditions. We found that an antigen concentration of 0.5 micrograms per lane in concert with ovalbumin blocking of the nitrocellulose...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Principais autores: Lange, R, Bocklage, H, Schneider, T, Kölmel, H W, Heesemann, J, Karch, H
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1992
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265029/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1734059/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.1.229-232.1992
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