Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography

BACKGROUND: During the past twelve years, lentiviral (LV) vectors have emerged as valuable tools for transgene delivery because of their ability to transduce nondividing cells and their capacity to sustain long-term transgene expression in target cells in vitro and in vivo. However, despite signific...

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Hlavní autoři: Kutner, Robert H, Puthli, Sharon, Marino, Michael P, Reiser, Jakob
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2009
Témata:
Methodology Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2649911/
https://ncbi.nlm.nih.gov/pubmed/19220915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-10
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