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Direct In-Gel Fluorescence Detection and Cellular Imaging of O-GlcNAc-Modified Proteins
[Image: see text] We report an advanced chemoenzymatic strategy for the direct fluorescence detection, proteomic analysis, and cellular imaging of O-GlcNAc-modified proteins. O-GlcNAc residues are selectively labeled with fluorescent or biotin tags using an engineered galactosyltransferase enzyme an...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2649877/ https://ncbi.nlm.nih.gov/pubmed/18683930 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja8030467 |
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