Direct In-Gel Fluorescence Detection and Cellular Imaging of O-GlcNAc-Modified Proteins

[Image: see text] We report an advanced chemoenzymatic strategy for the direct fluorescence detection, proteomic analysis, and cellular imaging of O-GlcNAc-modified proteins. O-GlcNAc residues are selectively labeled with fluorescent or biotin tags using an engineered galactosyltransferase enzyme an...

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Main Authors: Clark, Peter M., Dweck, Jessica F., Mason, Daniel E., Hart, Courtenay R., Buck, Suzanne B., Peters, Eric C., Agnew, Brian J., Hsieh-Wilson, Linda C.
Format: Artigo
Jezik:Inglês
Izdano: 2008
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2649877/
https://ncbi.nlm.nih.gov/pubmed/18683930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja8030467
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