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Tandem Fluorescent Proteins as Enhanced FRET-based Substrates for Botulinum Neurotoxin Activity

The light chain of botulinum neurotoxin A (BoNT/A-LC) is a zinc-metalloprotease that requires two extended exosites for optimal substrate binding and recognition of its intracellular target SNAP25. CFP and YFP connected through SNAP25 peptide (141–206) containing both exosites (CsY) has been used in...

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Detalhes bibliográficos
Main Authors: Pires-Alves, Melissa, Ho, Mengfei, Aberle, Karla K., Janda, Kim D., Wilson, Brenda A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2649687/
https://ncbi.nlm.nih.gov/pubmed/19168088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.toxicon.2008.12.016
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